Volume 6, Issue 2 (6-2020)                   J. Hum. Environ. Health Promot 2020, 6(2): 69-76 | Back to browse issues page


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Roshani Neshat R, Bimakr M, Ganjloo A. Effects of Binary Solvent System on Radical Scavenging Activity and Recovery of Verbascoside from Lemon verbena Leaves. J. Hum. Environ. Health Promot. 2020; 6 (2) :69-76
URL: http://zums.ac.ir/jhehp/article-1-310-en.html
1- Department of Food Science and Engineering, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Abstract:   (466 Views)
Background: Verbascoside is the major biophenolic compound of Lemon verbena leaf. This study aimed to investigate the effects of the binary solvent system on the free radical scavenging activity (FRSA) and verbascoside recovery from L. verbena leaves, as well as the kinetic mass transfer of verbascoside.
Methods: Classic extraction was performed using various ratios of ethanol (EtOH) and water (H2O) (50:50-90:10% v/v). The FRSA was analyzed using spectrophotometric methods (2,2-diphenyl-1-picrylhydrazyl [DPPH] and hydroxyl radical [HO˙] assays). Reversed-phase high-performance liquid chromatography was used for the qualification and quantification of verbascoside, and the Peleg model described the kinetic mass transfer of verbascoside.
Results: The hydroethanolic solvent was composed of EtOH:H2O (80:20% v/v) as the optimal medium for the maximum recovery of verbascoside (19.20 ± 0.12 mg/g) and FRSA of the extracts (45.25 ± 0.95% DPPHsc% and 31.17 ± 1.20% HOsc%). The Peleg model had a proper fit for the observed data with the highest coefficient of determination (R2 =0.999), the lowest root mean square error (RMSE = 0.093), and the mean relative percentage deviation modulus (E = 0.968).
Conclusion: The valuable bioactive compounds of L. verbena could be successfully extracted using the binary solvent system. The Peleg model is also an efficient non-linear model to describe the verbascoside release rate during extraction.
Full-Text [PDF 801 kb]   (216 Downloads)    
Type of Study: Research Article | Subject: Food Safety and Hygiene
Received: 2020/04/4 | Accepted: 2020/05/27 | Published: 2020/06/30

References
1. Gonzalez S. Dietary Bioactive Compounds and Human Health and Disease. Nutrients. 2020; 12: 348-50. [Crossref]
2. Dabeek WM, Marra MV. Dietary Quercetin and Kaempferol: Bioavailability and Potential Cardiovascular Related Bioactivity in Humans. Nutrients. 2019; 11: 2288- 307. [Crossref]
3. Soltani B, Rostami Asrabadi R, Shahpiri P, Marques Z, Rahimi AM, Farzaei MH. Aloysia Citrodora Palau (Lemon verbena): A Review of Phytochemistry and Pharmacology. J Ethnopharmacol. 2018; 222: 34-51. [Crossref]
4. Leyva Jimenez FJ, Lozano Sanchez J, Borras Linares I, Arraez Roman D, Segura Carretero A. Comparative Study of Conventional and Pressurized Liquid Extraction for Recovering Bioactive Compounds from Lippia Citriodora Leaves. Food Res Int. 2018; 109: 213-22. [Crossref]
5. Altemimi A, Lightfoot DA, Kinse M, Watson DG. Employing Response Surface Methodology for the Optimization of Ultrasound Assisted Extraction of Lutein and β-Carotene from Spinach. Molecules. 2015; 20: 6611-25. [Crossref]
6. Kronholm J, Revilla Ruiz P, Porras SP, Hartonen K, Carabias Martinez R, Riekkola ML. Comparison of Gas Chromatography Mass Spectrometry and Capillary Electrophoresis in Analysis of Phenolic Compounds Extracted from Solid Matrices with Pressurized Hot Water. J Chromatogr A. 2004; 1022: 9-16. [Crossref]
7. Luque de Castro MD, Priego Capote F. Soxhlet Extraction: Past and Present Panacea. J Chromatogr A. 2010; 1217: 2383-89. [Crossref]
8. Yen GC, Chen HY. Antioxidant Activity of Various Tea Extracts in Reletion to their Antimutagenicity. J Agr Food Chem. 1995; 43: 27-32. [Crossref]
9. Poodi Y, Bimakr M, Ganjloo A, Zarringhalami S. Intensification of Bioactive Compounds Extraction from Feijoa (Feijoa Sellowiana) Leaves using Ultrasonic Waves. Food Bioprod Process. 2018; 108: 37-50. [Crossref]
10. Fernandez L, de Santos J, Diaz Lanza AM, Perez E, Villaescusa L. Quantitative Determination of Verbascoside in Scrophularia Scorodonia by High-Performance Liquid Chromatography. Pharm Biol. 2005; 43: 226-29. [Crossref]
11. Shirsath SR, Sable SS, Gaikwad SG, Sonawane SH, Saini DR, Gogate PR. Intensification of Extraction of Curcumin from Curcuma Amada using Ultrasound Assisted Approach: Effect of Different Operating Parameters. Ultrason Sonochem. 2017; 38: 437-45. [Crossref]
12. Lau CH, Chua LS, Lee CT, Aziz R. Optimization and Kinetic Modeling of Rosmarinic Acid Extraction from Orthosiphon Stamineus. Curr Bioact Compd. 2014; 10: 271-85. [Crossref]
13. Bajpai VK, Baek KH, Kang SC. Antioxidant and Free Radical Scavenging Activities of Taxoquinone, A Diterpenoid Isolated from Metasequoia Glyptostroboides. S Afr J Bot. 2017; 111: 93-8. [Crossref]
14. Boulekbache Makhlouf L, Medouni L, Medouni Adrar S, Arkoub L, Madana K. Effect of Solvents Extraction on Phenolic Content and Antioxidant Activity of the Byproduct of Eggplant. Ind Crops Prod. 2013; 49: 668-74. [Crossref]
15. Ma JS, Liu H, Han CR, Zeng SJ, Xu XJ, Lu DJ, et al. Extraction, Characterization and Antioxidant Activity of Polysaccharide from Pouteria Campechiana Seed. Carbohydr Polym. 2020; 229: 115409. [Crossref]
16. Bandyopadhyay U, Das D, Banerjee RK. Reactive Oxygen Species: Oxidative Damage and Pathogenesis. Curr Sci. 1999; 10(77): 658-66.
17. Kardag A, Ozcelik B, Saner S. Review of Methods to Determine Antioxidant Capacities. Food Anal Methods. 2009; 13: 41-60. [Crossref]
18. Mukhopadhyay D, Dasgupta P, Sinha Roy D, Shauroseni A. Sensitive In Vitro Spectrophotometric Hydrogen Peroxide. Free Radicals and Antioxidants. 2016; 6: 124-32. [Crossref]
19. Locatelli M, Gindro R, Travaglia F, Coisson JD, Rinaldi M. Study of the DPPH-Scavenging Activity: Development of a Free Software for the Correct Interpretation of Data. Food Chem. 2009; 114: 889-97. [Crossref]
20. Carocho M, Morales P, Ferreira ICFR. Antioxidants: Reviewing the Chemistry, Food Applications, Legislation and Role as Preservatives. Trends Food Sci Technol. 2018; 71: 107-20. [Crossref]
21. Maqsood S, Adiamo O, Ahmad M, Mudgi P. Bioactive Compounds from Date Fruit and Seed as Potential Nutraceutical and Functional Food Ingredients. Food Chem. 2020; 38: 125522. [Crossref]
22. Waszkowiak K, Gliszczynska Swiglo A. Binary Ethanol-Water Solvents Affect Phenolic Profile and Antioxidant Capacity of Flaxseed Extracts. Eur Food Res Technol. 2016; 242: 777-86. [Crossref]
23. Cadiz Gurrera ML, Olivares Vicente M, Herranz Lopez M, Arraez Roman D, Fernandez Arroyo S, Micol V, et al. Bioassay-Guided Purification of Lippia Citriodora Polyphenols with AMPK Modulatory Activity. J Funct Foods. 2018; 46: 514-20. [Crossref]
24. Taha FS, Mohamed GF, Mohamed SH, Mohamed SS, Kamil MM. Optimization of the Extraction of Total Phenolic Compounds from Sunflower Meal and Evaluation of the Bioactivities of Chosen Extracts. Am J Food Technol. 2011; 6:1002-20. [Crossref]
25. Wettasinghe M, Shahidi F. Antioxidant and Free Radical Scavenging Properties of Ethanolic Extracts of Defatted Borage (Borago Officinalic L.) Seeds. Food Chem. 1999; 67: 399-414. [Crossref]
26. Zhang ZS, Li D, Wang LJ, Ozkan N, Chen XD, Mao ZH, et al. Optimization of Ethanol-Water Extraction of Lignans from Flaxseed. Sep Purif Technol. 2007; 57: 17-24. [Crossref]
27. Abramovic H, Grobin B, Ulrih NP, Cigic B. Relevance and Standardization of in Vitro Antioxidant Assays: ABTS, DPPH, and Folin-Ciocalteu. J Chem. 2018; 1-9. [Crossref]
28. Bimakr M, AR Russly, Ganjloo A, Food Processing and Impact on Active Components in Foods. CRC Press. 2015: 407-12. [Crossref]
29. Thaipong K, Boonprakob U, Crosby K. Cisneros Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC Assays for Estimating Antioxidant Activity from Guava Fruit Extracts. J Food Compost Anal. 2006; 19: 669-75. [Crossref]
30. Goulas V, Gomez Caravaca AM, Exarchou V, Gerothanassis IP, Segura Carretero A, Fernández Gutiérrez A. Exploring the Antioxidant Potential of Teucrium polium Extracts by HPLC-SPE-NMR and On-line Radical-Scavenging Activity Detection. LWT Food Sci and Technol. 2012; 46: 104-9. [Crossref]
31. Saifullah Md, McCullum R, McCluskey A, Vuong Q. Comparison of Conventional Extraction Technique with Ultrasound Assisted Extraction on Recovery of Phenolic Compounds from Lemon Scented Tea Tree (Leptospermum petersonii) Leaves. Heliyon. 2020; 6: e03666. [Crossref]
32. Zhao C, Li C, Liu S, Yang L. The Galloyl Catechins Contributing to Main Antioxidant Capacity of Tea Made from Camellia sinensis in China. Sci World J. 2014; 863984. [Crossref]
33. Hazwan MH, Hasfalina CM, Hishamuddin J, Zurina ZA. Optimization and Kinetics of Essential Oil Extraction from Citronella Grass by Ohmic Heated Hydrodistillation. Int J Chem Eng Appl. 2012; 3(3): 1-6.
34. Pin KY, Chuah AL, Rashih AA, Rasadah MA. Solid Liquid Extraction of Betel Leaves (Piper betel L.). J Food Process Eng. 2011; 34: 549-65. [Crossref]
35. Sayyar S, Abidin ZZ, Yunus R, Muhammad A. Extraction of Oil from Jathropa Seeds Optimization and Kinetics. Am J Appl Sci. 2009; 6: 1390-5. [Crossref]
36. Salehi B, Armstrong L, Rescigno A, Yeskaliyeva B, Seitimova G, Beyatli A, et al. Lamium Plants A Comprehensive Review on Health Benefits and Biological Activities. Molecules. 2019; 24(10): 1913. [Crossref]

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